Dextranase yog dab tsi?
Dextranase yog ib chav kawm ntawm hydrolases uas tshwj xeeb hydrolyze -1,6 glycosidic bonds hauv dextran. Nws belongs rau glycoside hydrolase tsev neeg, thiab nws lub luag haujlwm tseem ceeb yog kom degrade siab -molecular- hnyav dextran rau hauv qis-molecular-weight oligosaccharides lossis monosaccharides. Dextranase tuaj yeem hloov cov dextran ntuj insoluble rau hauv daim ntawv soluble, yog li txhim kho nws cov nqi siv.
Qhov chaw thiab kev faib tawm ntawm Dextranase
Dextranase muaj ntau qhov chaw, nrog rau cov kab mob thiab cov tsiaj. Ntawm cov no, cov kab mob microorganisms yog qhov tseem ceeb rau kev tsim khoom lag luam tam sim no. Siab -yield hom ntawm Chaetomium gracile kuaj los ntawm mutagenesis thiab Aspergillus niger tuaj yeem ua kom muaj txiaj ntsig zoo ntawm dextranase.
Dextranase tau muab faib ua ob hom loj: endo-dextranase thiab exo-dextranase. Endo- hom enzymes randomly tawg glycosidic daim ntawv cog lus hauv dextran molecules, tawg sai sai tawg polysaccharides loj thiab txo qhov hnyav molecular thiab viscosity ntawm cov polymer. Exo- hom enzymes sequentially hydrolyze qabzib residues los ntawm qhov kawg ntawm dextran molecules, maj mam tso me me - cov khoom molecule, uas feem ntau yog qabzib lossis disaccharides.
Mechanism ntawm Kev Ua ntawm Dextranase
Qhov kev txiav txim ntawm dextranase yog qhov tseem ceeb ntawm enzyme -catalyzed hydrolysis cov tshuaj tiv thaiv. Nws lub vev xaib nquag muaj lub npe tshwj xeeb uas paub txog -1,6 glycosidic daim ntawv cog lus ntawm dextran. Thaum cov enzyme khi rau lub substrate, cov amino acid residues nyob rau hauv lub active site catalyze lub cleavage ntawm glycosidic daim ntawv cog lus, ua rau lub depolymerization ntawm lub siab -molecular-yuag dextran saw.
Cov txheej txheem tshwj xeeb yog raws li hauv qab no: cov enzyme molecule paub txog hom glycosidic daim ntawv cog lus thiab nws cov qauv nyob ib puag ncig hauv dextran molecule los ntawm cov amino acid residues hauv qhov chaw nquag. Lub chaw ua haujlwm tsim ib qho kev sib txuas nrog cov dextran substrate, muab cov glycosidic daim ntawv cog lus ntawm lub substrate molecule nyob rau hauv ib tug conformation uas yog yooj yim catalyzed. Los ntawm cov kua qaub-base catalysis lossis covalent catalysis, nws tawm tsam cov pa atom ntawm glycosidic daim ntawv cog lus thiab rhuav tshem cov ntawv cog lus. Tom qab glycosidic daim ntawv cog lus yog cleaved, me me - cov khoom molecule xws li oligosaccharides los yog qabzib yog tsim thiab dissociate los ntawm lub enzyme lub active site. Cov enzyme dawb tuaj yeem rov ua dua nrog cov dextran substrates tshiab kom rov ua cov txheej txheem catalytic.
Kev siv Dextranase hauv cov khoom noj yog dab tsi?
Hauv kev lag luam qab zib, dextranase tuaj yeem hydrolyze dextran hauv beet lossis cane kua txiv, txo cov viscosity ntawm cov kua qab zib, zam kev cuam tshuam ntawm dextran ntawm sucrose crystallization thaum lub sij hawm crystallization txheej txheem, thiab txhim kho cov efficiency thiab yield ntawm qab zib.
Hauv cov khoom noj ci, ntxiv qhov tsim nyog ntawm dextranase tuaj yeem txhim kho lub khob noom cookie zoo nkauj, txhim kho gluten ruaj khov, thiab txhim kho saj thiab txee lub neej ntawm qhob cij.
Dextranase kuj tseem siv tau rau cov kua txiv hmab txiv ntoo, tshem tawm turbidity tsim los ntawm dextran hauv cov kua txiv hmab txiv ntoo thiab txhim kho cov kua txiv hmab txiv ntoo.